Loop-Mediated Isothermal Amplification-Immunochromatography (LAMP-IC) Assay for Rapid Halal Food Authentication

Abstract

Rising consumer demand for healthy, contaminant-free food, along with increased interest in halal certification, has underscored the need for reliable food authentication methods. Loop-mediated isothermal amplification (LAMP) is a rapid and efficient DNA amplification method that has been widely applied in various fields, including halal food authentication. This chapter highlights the development of immunochromatographic (IC) strip tests and LAMP-based nucleotide detection combined with IC (LAMP-IC) as in-house halal authentication kits. IC strip tests were designed to detect pork adulteration in beef or chicken mixtures, achieving sensitivity up to 1/5,000–1/10,000 in raw samples, though they are currently ineffective for cooked foods. In contrast, LAMP-IC effectively detects dog and pig DNA in cooked meat (boiled at 100°C for 15 minutes) within a single reaction at 68°C for 30 minutes, followed by IC strip analysis in 5–30 minutes. These findings demonstrate LAMP-IC as a practical and promising tool for halal food authentication.