Determination of Bioactive Compounds, Biochemical and Antioxidant Properties of Selected Minor Indigenous Fruits in Bangladesh

Abstract

Minor fruits may contain antinutrients, but there is no comprehensive primary data available in Bangladesh. As a result, the current research was carried out in order to collect data for a database of minor fruit composition. The antioxidant activity of 1,1-diphenyl-2picryl hydrazyl (DPPH) scavenging and reducing power assays were used to evaluate the total phenolic (TPH), vitamin C, total carotene, and ß-carotene contents and antioxidant activity of selected minor fruits (RPA). High-performance liquid chromatography with a photodiode array detector and autosampler was used to analyse phenolic compounds. The findings revealed that minor fruits contain a variety of phytochemicals,, particularly TPH, ascorbic acid, total flavonoid (TF), ß-carotene, total carotenoid (TC), and total anthocyanin content (TAC); values ranged, respectively, 0.23-176.50 mg GAE/g, 16.67-664.92 mg/100 g, 2.26-150.02 mg QE/100 g, 1.41-6897.57 µg/100 g, 1.26-98.24 mg/100 g and 1.15-47.46 mg/100 g. In the parameters antioxidant activity, total antioxidant capacity, DPPH, reducing power capacity (RPC), ferric reducing antioxidant power (FRAP), metal chelating capacity (MCC), nitric oxide (NO), and free radical scavenging activity, IC₅₀ ranged 0.01-278.24 µg of ascorbic acid/mg of extract, 39.70-250.00%, 3.21-634.00%, 0.02-1817.88 µM Fe2SO4/100g, 22.29-210.43%, 0.02-70.50%, and 4.98-856.70 µg/g, respectively. Phenolic acids that have been found and quantified, leading examples were gallic acid (279.06 mg/100 g), vanilic acid (43.77 mg/100 g), Þ-courmaric acid (178.96 mg/100 g), ferulic acid (20.44 mg/100 g), and lutein (91.13 µg/100 g) in aonla, day fruit, elephant apple, and bilimbi. Furthermore, all of the minor fruits are high in bioactive, biochemical, and antioxidant compounds that could be used in therapeutic applications.