Study on Proteomic Profiling of High-grade Glioblastoma: Aspects of Biomarkers

Authors

  • Stanislav N. Naryzhny Institute of Biomedical Chemistry, Pogodinskaya 10, Moscow, 119121, Russia and B. P. Konstantinov Petersburg Nuclear Physics Institute, National Research Center "Kurchatov Institute", Leningrad Region, Gatchina, 188300, Russia.
  • Maria A. Maynskova Institute of Biomedical Chemistry, Pogodinskaya 10, Moscow, 119121, Russia.
  • Victor G. Zgoda Institute of Biomedical Chemistry, Pogodinskaya 10, Moscow, 119121, Russia.
  • Natalia L. Ronzhina B. P. Konstantinov Petersburg Nuclear Physics Institute, National Research Center "Kurchatov Institute", Leningrad Region, Gatchina, 188300, Russia.
  • Svetlana E. Novikova Institute of Biomedical Chemistry, Pogodinskaya 10, Moscow, 119121, Russia.
  • Natalia V. Belyakova B. P. Konstantinov Petersburg Nuclear Physics Institute, National Research Center "Kurchatov Institute", Leningrad Region, Gatchina, 188300, Russia.
  • Olga A. Kleyst B. P. Konstantinov Petersburg Nuclear Physics Institute, National Research Center "Kurchatov Institute", Leningrad Region, Gatchina, 188300, Russia.
  • Olga K. Legina B. P. Konstantinov Petersburg Nuclear Physics Institute, National Research Center "Kurchatov Institute", Leningrad Region, Gatchina, 188300, Russia.
  • Rimma A. Pantina B. P. Konstantinov Petersburg Nuclear Physics Institute, National Research Center "Kurchatov Institute", Leningrad Region, Gatchina, 188300, Russia.
  • Michael V. Filatov B. P. Konstantinov Petersburg Nuclear Physics Institute, National Research Center "Kurchatov Institute", Leningrad Region, Gatchina, 188300, Russia.

DOI:

https://doi.org/10.9734/bpi/cacb/v3/1688F

Keywords:

Proteome, proteoforms, two-dimensional electrophoresis, mass spectrometry, biomarkers, glioblastoma

Abstract

Identification and quantitative analysis of different proteoforms (protein species) presented in a cell line generated from high grade glioblastoma was performed using two-dimensional electrophoresis (2DE), mass spectrometry (ESI LC-MS/MS), and immunodetection. A 2DE protein map containing an extensive data set comprising 937 spots with 1542 unique protein identifications (proteoforms) of 600 genes was obtained. Additionally, another set of experiments was performed where 16012 proteoforms coded by 4050 genes were identified by MS/MS according to their position in 96 gel sections (pixels). A special attention has been paid to the proteins that are the potential biomarkers of glioblastoma. The list of these biomarkers was compiled from literature. Next, we generated the graphs with theoretical and experimental information about proteoforms coded by the same gene. Such a virtual-experimental representation allowed better visualization of the state of these gene products. Many proteins, potential biomarkers of glioblastoma as well, are characterized by high numbers of protein species. We assume that these species could be a potential source of highly specific biomarkers of glioblastoma.

Published

2021-03-04

How to Cite

Stanislav N. Naryzhny, Maria A. Maynskova, Victor G. Zgoda, Natalia L. Ronzhina, Svetlana E. Novikova, Natalia V. Belyakova, … Michael V. Filatov. (2021). Study on Proteomic Profiling of High-grade Glioblastoma: Aspects of Biomarkers. Current Advances in Chemistry and Biochemistry Vol. 3, 112–128. https://doi.org/10.9734/bpi/cacb/v3/1688F

Issue

Current Advances in Chemistry and Biochemistry Vol. 3

Section

Chapters