An In-vitro and In-vivo Study of Antioxidant Potential of Green Synthesized Flavonoid Loaded Mono and Bimetallic Nanoparticles

Abstract

Remarkable success of nanotechnology has un-wrapped the visualization for developing mono and multimetallic nanoparticles exhibiting a wide range of applications because of their unique behavior. Gold and silver nanoparticles have received significant progress particularly in the area of biomedical research. The study demonstrates a single step environmentally benign approach to fabricate mono (Au, Ag) and bimetallic (Au-Ag) nanoparticles using flavonoids, extracted from the bark of the plant Madhuca longifolia. The effect of the architecture has been studied on the behavior of mono (Au, Ag) and bimetallic (Au-Ag) nanoparticles towards antioxidant bio-efficacy. Bimetallic (Au-Ag) nanoparticles have persuaded comparatively higher antioxidant bio-efficacy than monometallic nanoparticles. Based on our experiments and relevant information, a tentative mechanism has been suggested for the higher antioxidant bio-efficacy of bimetallic nanoparticles. The capping of medicinally important flavonoids on the freshly generated metal nanoparticles for the enhancement in the target bio-efficacy has been proved using HPTLC fingerprinting. The proposed green nanotechnological strategy for the fabrication of bimetallic nanoparticles seems to be an excellent approach for the enhancement in the antioxidant bio-efficacy without performing synthetic structural modifications.