Intervention Effect of Malus pumila Leaf Flavonoids on Sennainduced Acute Diarrhea in BALB/c Mice

Authors

  • Ruokun Yi Chongqing University of Education, China.
  • Yu Tian Department of Critical Care Medicine, The First Affiliated Hospital of Chengdu Medical College, Chengdu, China.
  • Fang Tan Department of Public Health, Our Lady of Fatima University, Valenzuela, Philippines.
  • Wenfeng Li School of Life Science and Biotechnology, Yangtze Normal University, Chongqing, China.
  • Jianfei Mu Chongqing University of Education, China.
  • Xingyao Long Chongqing University of Education, China.
  • Yanni Pan Chongqing University of Education, China.
  • Xin Zhao Chongqing University of Education, China.

Keywords:

Acute diarrhea, aquaporin, expression, flavonoid, Malus pumila leaf

Abstract

The Malus pumila leaves are used as a kind of tea drink in China, and there are abundant flavonoids in the leaves of Malus pumila. In this study, BALB/c mice received senna extract solution via gavage, which induced acute diarrhea, and the interventive effect of Malus pumila leaf flavonoids (MPLF) was observed. The results showed that MPLF decreased the diarrhea index, and MPLF also decreased the capillary permeability in the abdominal cavity of mice. The result of serum cytokine detection indicated that MPLF decreased the levels of inflammatory cytokines, including interleukin 6 (IL-6), interleukin 12 (IL-12), and tumor necrosis factor alpha (TNF-\(\alpha\)). The results of quantitative polymerase chain reaction (qPCR) indicated that diarrhea decreased the mRNA and protein expression of aquaporin-3 (AQP3) in the jejunum and aquaporin-4 (AQP4) in the ileum, which were inhibited by MPLF. By high performance liquid chromatography (HPLC), MPLF mainly contains 10 flavonoids, which are rutin, hyperoside, isoquercitrin, taxifolin, quercitrin, hesperidin, myricetin, baicalin, neohesperidin dihydrochalcone and quercetin, the synergistic effect of these components plays an antidiarrhea role in MPLF. Therefore, MPLF achieved good antidiarrheal effect, which was better than that of the commonly used montmorillonite powder at the same concentration. MPLF is a natural raw material for functional food with an antidiarrheal effect.

Published

2021-07-29

How to Cite

Ruokun Yi, Yu Tian, Fang Tan, Wenfeng Li, Jianfei Mu, Xingyao Long, … Xin Zhao. (2021). Intervention Effect of Malus pumila Leaf Flavonoids on Sennainduced Acute Diarrhea in BALB/c Mice. Study on Bioactivity of Food and Drug Homologous Products, 70–81. Retrieved from https://stm.bookpi.org/SBFDHP/article/view/2713

Issue

Study on Bioactivity of Food and Drug Homologous Products

Section

Contents