Toxic Chemical-Free Mobile Phase HPLC Determination of Malachite Green and Leuco-malachite Green

Abstract

Using an isocratic hazardous organic solvent/reagent-free mobile phase, this chapter describes a reserved-phase high-performance liquid chromatographic (HPLC) method for detecting malachite green (MG) and leucomalachite green (LMG). Eliminating the use of toxic solvents and reagents is an important goal in terms of environmental conservation, human health and the economy. Chromatographic separations were performed an Inertsil® WP300 C4 with 0.02 mol/L octane sulfonic acid-ethanol mobile phase and a photodiode-array detector. The overall running time was about 5 minutes. The suitability of the system met all of the international acceptance standards. A safe approach for simultaneously detecting MG and LMG has been discovered, and it could be used to quantify meals in the future.