Determining the Bioactive Constituents in Calotropis gigantea Leaves by GC-MS, HPLC and FTIR Techniques

Abstract

Medicinal plants have initiated in many countries because of their contributions to health care.  Calotropis gigantea is a biological ingredient with potential therapeutic qualities that belongs to the Asclepiadaceae family.Calotropis gigantea leaves have long been used to treat abdominal pain, tumours, skin problems, wounds, and insect stings.This plant's therapeutic characteristics make it an important source of a medicinal compound. Calotropis gigantean's bioactive components were studied using GC-MS, HPLC, and FTIR. Gas Chromatography-Mass Spectroscopy (GC-MS) study reveals major bioactive chemicals. Only leaves contained the chemical compounds Androstane-11,17-Dione, 3-[(Trimethylsilyl)Oxy]-, 17-[O-(Phenylmethyl)Oxime], 3.Alpha.-(Trimethylsiloxy)Cholest-5-Ene, Urs-12-EN-28-Oic acid, 3-Hydroxy-, Methyl ester, (3.BETA.)-, Pseduosarsasapogenin-5, 20-Dien Methyl Ether, Beta. Carotene, 1.Alpha.,2.Alpha.-Epoxy-1.Beta.-Methylcholesta-4,6-Dien-3-One, 3-O-Acetyl-6-Methoxy-Cycloartenol  whereas the rest of the compounds were similar in plants.