Determination of Residual Acetamiprid and Imidacloprid in Unpolished Rice by Centrifugal Spin Column Clean-up and Water Mobile Phase HPLC-Diode Array

Abstract

This chapter introduces a suitable analysis method for routine residual monitoring of antimicrobial drugs in food.  A rapid, simple, and space-saving technique of sample preparation followed by a 100% water mobile phase high-performance liquid chromatography (HPLC) coupled photo-diode array detector (PDA) for quantifying acetamiprid (ATP) and imidacloprid (ICP) in unpolished rice was described here. The analytes were extracted from the sample using a handheld ultrasonic homogenizer with water, and purified by MonoSpin® C18-CX, a centrifugal monolithic silica spin mini-column, and quantified within 20 min sample^-1.  The accuracy, precision, and system suitability are well within the international method acceptance criteria.