Analytical Assay of Betamethasone Pharmaceutical Dosage Forms by Visible Spectrophotometry

Abstract

Two simple and reproducible methods have been developed and validated for the determination of betamethasone in pharmaceutical dosage forms by visible spectrophotometry. Method-1 is based on the formation of meisenheimer like sigma -complex by the m-dinitrobenzene with the mentioned drug in presence of alkaline medium to from a colored chromogen measured at 490nm. The Method-2 is based on reaction between MBTH and drug under study in presence of alkali to from a colored chromogen measured at 620 nm. The findings of the investigation were statistically evaluated and recovery studies supported the correctness of the proposed approaches. The methods were successfully applied to the determination of betamethasone in pharmaceutical formulations.