Hemolytic Activity and Antibacterial Investigation against Human Pathogen: Fish Epidermal Mucus from Labeo rohita

Abstract

The fish mucus was extracted from Labeo rohita by using aqueous solution and the chemical composition showed 27.5% of protein, 5.8% of carbohydrate and 0.21% of lipids. The fish mucus contains a variety of biologically active compounds such as lysozyme, lectins, proteolytic enzymes, flavoenzymes, immunoglobulins, C-reactive protein, apolipoprotein A-I. The haemolytic activity of the mucus extract showed 43.47 and 37.73 HT/mg in human blood. Further the antibacterial activity of the mucus extract showed against S. aureus, K. pneumoniae, S. typhi, V. cholerae, K. oxytoca, E. coli, V. parahaemolyticus and S. pyogenes were 14, 12, 15, 16, 11, 17, 13 and 12mm at the highest concentration of 100µg/ ml. The fish mucus observed MIC values of 100µg/ml against E. coli and the V. cholerae slightly arrested at the above concentration. The fish mucus showed the MBC values of 100µg/ml against E. coli and the V. cholerae slightly arrested at the same concentration. The above antibacterial activity of the mucus suggested as a source antimicrobial agent in future pharmacological for the development of new antimicrobial drugs.