Changes in Gut Microorganism in Patients with Positive Immune Antibodies Associated Recurrent Abortion: An Advanced Study

Abstract

Background: This study aimed to analyze the changes in gut microorganism of patients with positive immune antibody associated recurrent abortion using 16s rRNA gene sequencing microbiome assay.

Methods: The fecal samples from 20 recurrent abortion women with positive immune antibody (positive group) and 20 with negative immune antibody (negative group) were collected. After 16s rRNA gene sequencing, the obtained raw reads were performed quality filtering to obtain the clean tags and then classified into microbial genomes. All effective tags were clustered into operational taxonomic units (OTUs) and the representative sequence was selected for the annotation of taxonomic information, followed by alpha and beta diversity analyses.

Results: A total of 43,116 OTUs were obtained in all 40 samples. Bacteroides had the highest relative abundance in the positive group. In the negative group, Bacteroides, Erysipelotrichaceae_UCG-003, Faecalibacterium and Prevotella_9 had high relative abundance. Alpha diversity analysis results showed that the community richness, community diversity, and phylogenetic diversity in the positive group were higher than that in the negative group. Prevotella_9, Enterococcus, Megasphaera and Anaerostipes presented significant differences between negative and positive groups.

Conclusion: The present study for the first time investigated the gut microbiome involved in positive immune antibody associated recurrent abortion via 16s rRNA gene sequencing microbiome assay. The genera that were significantly differential between positive and negative groups may serve as therapeutic targets for positive immune antibody associated recurrent abortion.