5-[Substituted]-1, 3, 4-thiadiazol-2-amines: Synthesis, Spectral Characterization, and Evaluation of their DNA Interactions

Abstract

Synthesis of novel thiadiazoles is one of the important aspects in the development of therapeutic drugs. Emphasizing the properties of the new compounds having potential novel structural features and biological applications have been current research in medicinal chemistry. In this connection, here reporting the synthesis and characterization of 5-[substituted]-1, 3, 4-thiadiazol-2-amines (1-7). All the prepared compounds were characterized by spectroscopic methods viz.¹H-NMR, ¹³C{¹H}-NMR, FT-IR, and LC-MS. The DNA binding interactions of the 1,3,4-thiadiazoles were undertaken by absorption and fluorescence spectroscopy methods. The results reveal that the compounds are avid binders to DNA via. Groove binding mode. The DNA cleavage studies of the compounds were carried out in presence and absence of H₂O₂ using gel electrophoresis.