Evaluation of Biofilm Formation by Stenotrophomonas maltophilia SRS05-GRD

Abstract

Biofilm formation is triggered by unfavourable external conditions that modify the expression of several genes. Biofilm, in turn, alters the microenvironment of its inhabitants that leads to alteration of gene expression and maturation of biofilm.  Stenotrophomonas maltophilia is an aerobic, glucose non-fermentative, Gram-negative bacterium wide spread in the environment. The present study reports the isolation, identification, genotypic and phenotypic characterization, of the Stenotrophomonas strains isolated from the industrial effluent sample and the ability of Stenotrophomonas maltophiliaSRS05-GRD (KF558319) for biofilm formation is evaluated using different substrates and different medium. Based on the graphical representation of optical density values obtained by incorporating various substrates in Tryptone Soya Broth (TSB), glass was found to support high biofilm formation of  Stenotrophomonas maltophilia SRS05-GRD. The results obtained indicate that the commercial media (TSB with glucose and soybean meal at pH 7) shows optimum biofilm formation. This study has, therefore, confirmed the potential of Stenotrophomonas maltophilia SRS05-GRD to form biofilm. Optimum medium and dilution of the culture was also determined that yield effective biofilm formation.