Combined Vaccine and Vaccine Interference: Lapin Immune Features

Abstract

A prototype candidate monotypic and combined vaccines [balance ,x-x and unbalance 2x-x] have been developed from the gram negative uro-pathogen E .coli and P. aeruginosa. These bacterins were applied for specific immune priming of rabbits using multisite injection protocols in a week a part dosing for two weeks followed by a week leave then bled in a homologous prime boost schedules. .Secondary immune response were tempted. The tripartite arms of the immune responses were investigated as. TNF alpha, bone marrow mitotic index, splenic body index, IL17 cytokine, antibody titers, cytokine IL4, IL2 and IL10. Combined bacterins are known to exhibit immune interference phenomena. The phenomenology of immune interference in bacterin combinations appeared as; one enhance the other, one dampened the other and one does not affect the other. E.coli were found to be of major and minor immune interfering potentials depending on the nature of bacterin combinations in a sense of quantitative ratios of the bacterin units per unit volume of the developed biologics .Both of the immune enhancing and immune inhibiting forms of the immune interference were noted. The developed uro-combined bacterin seems to be a novel contribution in continnum with the licensed urnum, urovac and urovaxum bacterins.