Biochemical pH Buffer Standards of the Zwitterionic Buffer TES (N-Tris-(Hydroxymethyl) Methyl- 2-Aminoethanesulfonic Acid) from \(5^{\circ}\)C to \(55^{\circ}\)C

Authors

  • Rabindra N. Roy Hoffman Department of Chemistry, Drury University, Springfield, USA.
  • Lakshmi N. Roy Hoffman Department of Chemistry, Drury University, Springfield, USA.
  • Blake M. Bodendorfer Hoffman Department of Chemistry, Drury University, Springfield, USA.
  • Zachary M. Downs Hoffman Department of Chemistry, Drury University, Springfield, USA.
  • Stephen D. Rocchio Hoffman Department of Chemistry, Drury University, Springfield, USA.
  • Joshua T. Wollen Hoffman Department of Chemistry, Drury University, Springfield, USA.
  • Jessica M. Stegner Hoffman Department of Chemistry, Drury University, Springfield, USA.
  • Isaac B. Henson Hoffman Department of Chemistry, Drury University, Springfield, USA.
  • Nicholas W. Grove Hoffman Department of Chemistry, Drury University, Springfield, USA.
  • Lauren A. Dieterman Hoffman Department of Chemistry, Drury University, Springfield, USA.

DOI:

https://doi.org/10.9734/bpi/stda/v5/3475

Keywords:

Ionic strength, liquid junction potentials, second dissociation constant, buffer solutions, activity coefficients

Abstract

A selected group of zwitterionic buffers has been proposed for physiological use. The authors have undertaken the determination of pH values for one buffer solution of TES without NaCl and nine buffer solutions with NaCl yielding an ionic strength I = 0.16 mol \(\cdot\) kg-1 similar to that of blood. The TES was obtained from Research Organics in Cleveland, OH. The experimental method for further crystallization and the assay have been previously reported in detail. These buffer solutions have been evaluated in the temperature range of \(5^{\circ}\)C to \(55^{\circ}\)C using an extended version of the Debye-Hückel equation. The pH values are reported using 1) the Debye-Hückel extension of the Bates-Guggenheim convention in the temperature range \(5^{\circ}\)C to \(55^{\circ}\)C and 2) with and without liquid junction correction at \(25^{\circ}\)C and \(37^{\circ}\)C . These TES buffer solutions are recommended as secondary standard references for pH measurements in the range of pH 7.2 to 7.5 for physiological applications with an ionic strength of I = 0.16 mol \(\cdot\) kg-1 .

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Published

2025-02-07

How to Cite

Rabindra N. Roy, Lakshmi N. Roy, Blake M. Bodendorfer, Zachary M. Downs, Stephen D. Rocchio, Joshua T. Wollen, … Lauren A. Dieterman. (2025). Biochemical pH Buffer Standards of the Zwitterionic Buffer TES (N-Tris-(Hydroxymethyl) Methyl- 2-Aminoethanesulfonic Acid) from \(5^{\circ}\)C to \(55^{\circ}\)C. Science and Technology: Developments and Applications Vol. 5, 15–28. https://doi.org/10.9734/bpi/stda/v5/3475

Issue

Science and Technology: Developments and Applications Vol. 5

Section

Chapters