Identification of Low and High Erucic Acid Containing Genotype(S) in Indian Mustard Employing Molecular Markers

Abstract

In traditional Brassica oilseeds, the incidence of erucic acid is well-thought-out as anti-nutritional issue for human ingesting as these reasons toxic possessions on the heart at higher enough dosages. As such there is a pressing necessity to restrain the erucic acid content and develop varieties having low erucic acid through conventional and/or molecular breeding approaches. Genetic enhancement of crops can be augmented when there is a broad genetic variation and information on these genetic possessions is available. The current investigation was carried out with 48 Brassica genotype (s) with the intention to recognize genotype (s) with low and high erucic acid content on the basis of molecular markers. For the investigation, a total of 50 SSR molecular markers were chosen for the amplification of genomic DNA. Out of these, only 23 SSR molecular markers were found to be polymorphic. A total of 109 alleles were recognized with an average of 4.47 alleles per locus for polymorphic SSR markers. Genetic diversity ranged between 0.55 for marker Na10-D07 to 0.77 for marker BRMS-098 with a mean worth of 0.68. Polymorphism information content (PIC) value of the markers varied from 0.51 for SSR Na10-D07 to 0.73 for the primer BRMS-098 with an average value of 0.62. The dendrogram was constructed and major three clusters were formed. Most of the genotypes were clustered conferring to the sites they established. Genotypes evidenced with low erucic acid content displayed higher similarity and grouped together. Mustard genotypes recognized with higher genetic variability with convenient traits may be employed for crop improvement programmes in future.