Plant Genomic DNA Isolation: An Important Technology for Marker Assisted Selection

Abstract

Plant biotechnology is necessary to maximize the probability of success in crop improvement programs. One area of biotechnology is DNA marker technology, derived from research in molecular genetics and genomics, offers great promise for plant breeding. Marker assisted selection in plant breeding, increase efficiency and precision. Mapping and marker assisted selection of targeted trait require high quality of DNA from a large number of plants in short time. However, many protocols have been developed and reported for extraction of plant DNA with the primary aim of development of a relatively quick, inexpensive and consistent to extract high quality DNA. The general principle of all these DNA extraction protocols remains the same involving disruption of the cell wall, cell membrane and nuclear membrane to release the highly intact DNA into solution ensuring removal of the contaminating biomolecules such as the proteins, polysaccharides, lipids, phenols and other secondary metabolites by enzymatic or chemical methods. High quality DNA is prime requirement for marker assisted selection applications in crop improvement as well as sequencing-based applications. Current article compiled Plant Genomic DNA isolation and quantification protocols for different crops.