Study on Cloning, Sequencing and Phylogenetic Analysis of Granulocyte Macrophage Colony Stimulating Factor of Indian Cattle (Bos indicus)

Authors

  • Ramya Kalaivanan Veterinary College and Research Institute, Namakkal-02, India. and Indian Veterinary Research Institute, Hebbal, India.
  • P. Sankar Veterinary College and Research Institute, Namakkal-02, India.
  • Subodh Kishore Indian Veterinary Research Institute, Hebbal, India.

DOI:

https://doi.org/10.9734/bpi/raavs/v2/5204D

Keywords:

Granulocyte Macrophage colony stimulating factor, peripheral blood mononuclear cells, PCR, Cloning

Abstract

Reverse transcriptase polymerase chain reaction (RT-PCR) was used to amplify the cDNA coding for Granulocyte macrophage colony stimulating factor (GM-CSF) from LPS activated macrophages, which was then cloned into the pCNDA vector and sequenced. Without the secretory signal peptide sequence, the amplified bovine GM-CSF cDNA was 755 bp long. The 755-bp open reading frame (ORF) coded for 143 amino acids with a molecular weight of 16200 Da, and it was 99 and 96 percent identical to Bos taurus and Bubalus bubalis, respectively. The phylogenetic analysis revealed swine and equine GM-CSF sequences are most different from buffalo, sheep, goat, and deer.

Published

2021-10-01

How to Cite

Ramya Kalaivanan, P. Sankar, & Subodh Kishore. (2021). Study on Cloning, Sequencing and Phylogenetic Analysis of Granulocyte Macrophage Colony Stimulating Factor of Indian Cattle (Bos indicus). Research Aspects in Agriculture and Veterinary Science Vol. 2, 16–21. https://doi.org/10.9734/bpi/raavs/v2/5204D

Issue

Research Aspects in Agriculture and Veterinary Science Vol. 2

Section

Chapters