Zein Nanoplatforms for Functional Promotion of Pro-apoptosis and Antiproliferation Induced in Human Breast Carcinoma MCF7 Cells by Pterostilbene

Authors

  • Majid Mohammad Al-Sawahli Faculty of Pharmacy, Ahram Canadian University, 6th October City, Egypt, Department of Pharmaceutics, College of Pharmacy, The Islamic University, Najaf, Iraq and Department of Pharmaceutical Technology, Faculty of Pharmacy, Kafr Elsheikh University, Kafr Elsheikh, Egypt.
  • Mohamed A. Zarka Department of Pharmacognosy, College of Pharmacy, The Islamic University, Najaf, Iraq.
  • Mowafaq Mohammed Ghareeb Department of Pharmaceutics, College of Pharmacy, University of Baghdad, Baghdad, Iraq.
  • Hussam Ibrahim Kutbi Department of Pharmacy Practice, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia.
  • Ahmed Kamal Kammoun Department of Pharmaceutical Chemistry, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia.
  • Ayman M. Noreddin Department of Pharmacy Practice, Faculty of Pharmacy, Ahram Canadian University, 6th October City, Egypt and Department of Internal Medicine, School of Medicine, University of California, Irvine, California, USA.
  • Dalia Farag A. El-Telbany Department of Pharmaceutics, Faculty of Pharmacy, Modern University for Technology and Information (MTI), Cairo, Egypt.

DOI:

https://doi.org/10.9734/bpi/pramr/v12/9322F

Keywords:

Phototherapy, pterostilbene, MCF-7 cells, nanospheres, zein

Abstract

The aim of current study was to investigate potential of PTS-ZN NSs formulation on ameliorating cytotoxicity, pro-apoptotic and oxidative stress potentials of PTS in MCF-7 cells. PTS-ZN NSs were synthesised using the liquid-liquid phase separation method. The formulations developed were evaluated in terms of particle size analysis, encapsulation efficiency, and in vitro diffusion. Cytotoxicity, uptake, cell cycle progression, Annexin-V assay, apoptotic genes mRNA expression, and biochemical assays were also performed. Five formulations of PTS-ZN NSs (P1-P5) were prepared according to liquid–liquid phase separation method to diminish the use of solvents. The study indicated that apoptosis in MCF-7 cells was confirmed and augmented through caspases dependent signaling pathways and inhibition of CDK1 activity due to use of ZN-NSs in delivery of PTS in comparison with free PTS. Promising research outcomes included high EE% nanocomposites, 36 h extended PTS diffusion, a synergized cytotoxicity profile, enhanced MCF-7 cells uptake, ameliorated apoptosis induced potential, enhanced mRNA expression of CASP3, CASP7 and CDK1 genes and significant oxidative stress.

Published

2023-02-17

How to Cite

Majid Mohammad Al-Sawahli, Mohamed A. Zarka, Mowafaq Mohammed Ghareeb, Hussam Ibrahim Kutbi, Ahmed Kamal Kammoun, Ayman M. Noreddin, & Dalia Farag A. El-Telbany. (2023). Zein Nanoplatforms for Functional Promotion of Pro-apoptosis and Antiproliferation Induced in Human Breast Carcinoma MCF7 Cells by Pterostilbene. Perspective of Recent Advances in Medical Research Vol. 12, 93–114. https://doi.org/10.9734/bpi/pramr/v12/9322F

Issue

Perspective of Recent Advances in Medical Research Vol. 12

Section

Chapters