Lactobacillus plantarum KSFY06 Prevents Inflammatory Response and Oxidative Stress in Acute Liver Injury Induced by D-Gal/LPS in Mice

Authors

  • Chong Li Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing, 400067, People’s Republic of China and Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Chongqing 400067, People’s Republic of China and Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Chongqing 400067, People’s Republic of China.
  • Jun Si Pre-Hospital Emergency Department, Chongqing University Central Hospital, Chongqing Emergency Medical Center, Chongqing 400014, People’s Republic of China.
  • Fang Tan Department of Public Health, Our Lady of Fatima University, Valenzuela 838, Philippines.
  • Kun-Young Park Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing, 400067, People’s Republic of China and Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Chongqing 400067, People’s Republic of China and Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Chongqing 400067, People’s Republic of China.
  • Xin Zhao Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing, 400067, People’s Republic of China and Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Chongqing 400067, People’s Republic of China and Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Chongqing 400067, People’s Republic of China.

DOI:

https://doi.org/10.9734/bpi/mono/978-93-5547-256-4/CH9

Keywords:

Lactobacillus plantarum KSFY06, D-galactose, lipopolysaccharide, acute liver injury, mice

Abstract

Aim: The purpose of this study is to investigate the preventive effect of Lactobacillus plantarum KSFY06 (LP-KSFY06) on D-galactose/lipopolysaccharide (D-Gal/LPS)-induced acute liver injury (ALI) in mice.

Methods: We evaluated the antioxidant capacity of LP-KSFY06 in vitro, detailed the effects of LP-KSFY06 on the organ index, liver function index, biochemical index, cytokines, and related genes, and noted the accompanying pathological changes.

Results: The results clearly showed that LP-KSFY06 can remove 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzthiazoline - 6-sulphonic acid) diammonium salt (ABTS) free radicals in vitro. The analysis of the organ index and pathology demonstrated that LP-KSFY06 significantly prevented ALI. Biochemical and molecular biological analysis showed that LP-KSFY06 prevented a decrease in the antioxidant-related levels of superoxide dismutase (SOD), glutathione (GSH), glutathione peroxidase (GSH-Px), catalase (CAT), and total antioxidant capacity (T-AOC), and also prevented an increase in aspartate aminotransaminase (AST), alanine aminotransaminase (ALT), malondialdehyde (MDA), myeloperoxidase (MPO), and nitric oxide (NO) levels. LP-KSFY06 upregulated the anti-inflammatory factor interleukin (IL)-10 and downregulated the pro-inflammatory factors IL-6, IL-1\(\beta\), tumor necrosis factor-\(\alpha\) (TNF-\(\alpha\)), and interferon-\(\gamma\) (IFN-\(\gamma\) ). These oxidative and inflammatory indicators were consistent with the results of gene detections. Furthermore, we determined that LP-KSFY06 downregulated Keap1, NLRP3, apoptosis-associated speck-like protein containing a CARD (ASC), caspase-1, nuclear factor K-light-chain-enhancer of activated B cells (NF-KB), IL-18, and mitogen-activated protein kinase 14 (MAPK14 or p38), upregulated Nrf2, heme oxygenase-1 (HO-1), NAD(P)H dehydrogenase [quinone] 1 (NQO1), B-cell inhibitor-\(\alpha\) (IKB-\(\alpha\)), and thioredoxin (Trx) mRNA expression. These may be related to the regulation of the Kelch-like ECH-associated protein-1 (Keap1)-nuclear factor-erythroid-2-related factor (Nrf2)/antioxidant response element (ARE) and NLRP3/NF-KB pathways.

Conclusion: LP-KSFY06 is an effective multifunctional Lactobacillus with strong anti-oxidant and anti-inflammatory ability that can prevent D-gal/LPS-induced ALI in mice and assist in maintaining health.

Published

2022-02-21

How to Cite

Chong Li, Jun Si, Fang Tan, Kun-Young Park, & Xin Zhao. (2022). Lactobacillus plantarum KSFY06 Prevents Inflammatory Response and Oxidative Stress in Acute Liver Injury Induced by D-Gal/LPS in Mice. Probiotics and Oxidative Stress, 139–157. https://doi.org/10.9734/bpi/mono/978-93-5547-256-4/CH9

Issue

Probiotics and Oxidative Stress

Section

Content