Lactobacillus fermentum CQPC07 Attenuates Obesity, Inflammation and Dyslipidemia by Modulating the Antioxidant Capacity and Lipid Metabolism in High-fat Diet Induced Obese Mice

Authors

  • Ya Wu Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Xuefu Main Street 9 Nan’an District, Chongqing, 400067, People’s Republic of China and Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Xuefu Main Street 9 Nan’an District, Chongqing, 400067, People’s Republic of China and Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Xuefu Main Street 9 Nan’an District, Chongqing, 400067, People’s Republic of China and College of Biological and Chemical Engineering, Chongqing University of Education, Xuefu Main Street 9 Nan’an District, Chongqing, 400067, China.
  • Xueya Li Department of Dermatology, People’s Hospital of Chongqing Banan District, 659 Yunan Avenue, Longzhouwan Street, Banan District, Chongqing, 401320, China.
  • Fang Tan Department of Public Health, Our Lady of Fatima University, 838, Valenzuela, Philippines.
  • Xianrong Zhou Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Xuefu Main Street 9 Nan’an District, Chongqing, 400067, People’s Republic of China and Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Xuefu Main Street 9 Nan’an District, Chongqing, 400067, People’s Republic of China and Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Xuefu Main Street 9 Nan’an District, Chongqing, 400067, People’s Republic of China.
  • Jianfei Mu Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Xuefu Main Street 9 Nan’an District, Chongqing, 400067, People’s Republic of China and Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Xuefu Main Street 9 Nan’an District, Chongqing, 400067, People’s Republic of China and Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Xuefu Main Street 9 Nan’an District, Chongqing, 400067, People’s Republic of China.
  • Xin Zhao Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Xuefu Main Street 9 Nan’an District, Chongqing, 400067, People’s Republic of China and Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Xuefu Main Street 9 Nan’an District, Chongqing, 400067, People’s Republic of China and Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Xuefu Main Street 9 Nan’an District, Chongqing, 400067, People’s Republic of China.

DOI:

https://doi.org/10.9734/bpi/mono/978-93-5547-256-4/CH8

Keywords:

Lactobacillus fermentum, Anti-inflammation, High-fat diet, Anti-obesity, Lipid metabolism

Abstract

Background: Obesity is an epidemic disease in the world, the treatment and prevention of obesity methods have gained great attention. Lactobacillus is the main member of probiotics, and the physiological activity of it is specific to different strains. This study systematically explored the anti-obesity effect and possible mechanism of Lactobacillus fermentum CQPC07 (LF-CQPC07), which was isolated from pickled vegetables.

Results: LF-CQPC07 effectively controlled the weight gain of mice caused by a high-fat diet. The results of pathological sections indicated that LF-CQPC07 alleviated hepatocyte damage and fat accumulation in adipocytes. The detection of biochemical indictors revealed that LF-CQPC07 decreased the levels of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and triglycerides (TG), and increased the level of high-density lipoprotein cholesterol (HDL-C). Additionally, LF-CQPC07 caused the decrease in the amounts of inflammatory cytokines interleukin (IL)-1\(\beta\), tumor necrosis factor-\(\alpha\) (TNF-\(\alpha\)), IL-6, and interferon-\(\gamma\) (IFN-\(\gamma\)), and the increase in the amounts of the anti-inflammatory cytokines IL-10 and IL-4. LF-CQPC07 also decreased the amounts of alanine aminotransferase (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP). Confirmed by qPCR, LF-CQPC07 enhanced the mRNA expression of catalase (CAT), gamma glutamylcysteine synthetase 1 (GSH1), copper/zinc superoxide dismutase (SOD1), manganese superoxide dismutase (SOD2), and glutathione peroxidase (GSH-Px). It also increased the mRNA expression levels of carnitine palmitoyltransferase 1 (CPT1), peroxisome proliferator-activated receptor alpha (PPAR-\(\alpha\)), lipoprotein lipase (LPL), and cholesterol 7 alpha hydroxylase (CYP7A1), and decreased that of PPAR-\(\gamma\) and CCAAT/enhancer binding protein alpha (C/EBP-\(\alpha\)) in the liver of mice.

Conclusion: This research confirmed that LF-CQPC07 is capable of ameliorating obesity, improving hyperlipemia, and alleviating chronic low-grade inflammation and liver injury accompanied with obesity. Its mechanism may be the regulation of antioxidant capacity and lipid metabolism. Therefore, LF-CQPC07 has enormous potential to serve as a potential probiotic for the prevention or treatment of obesity.

Published

2022-02-21

How to Cite

Ya Wu, Xueya Li, Fang Tan, Xianrong Zhou, Jianfei Mu, & Xin Zhao. (2022). Lactobacillus fermentum CQPC07 Attenuates Obesity, Inflammation and Dyslipidemia by Modulating the Antioxidant Capacity and Lipid Metabolism in High-fat Diet Induced Obese Mice. Probiotics and Oxidative Stress, 123–138. https://doi.org/10.9734/bpi/mono/978-93-5547-256-4/CH8

Issue

Probiotics and Oxidative Stress

Section

Content