Gc-Ms Analysis of Cordia dichotoma G. Forst Bark Hexane Extract and Evaluation of Anti Bacterial Activity

Abstract

Aim: Cordia dichotoma G. Forst is a member of the Boraginaceae family and is well-known for its biological properties. The current study aims to investigate the antibacterial activity of stem bark hexane extract by analysing its phytoconstituents using GC-MS.

Objectives: The objectives include the extraction of plant bark’s crude using hexane as solvent, to know the presence of bio active compounds present in the hexane crude by using GC-MS technique. Purification of the crude hexane extract by column chromatography and by preparative HPLC. Further GC-MS analysis to know the active compounds present in the fractions obtained after purification. Isolation of \(\beta\)-Sitosterol and Stigmasterol. Assessment of Anti bacterial activity of the hexane crude.

Methods: The powdered stem bark was extracted with cold and hot hexane solvents, and the solvent from the extract was distilled off to be analysed by GC-MS. Saponification, column chromatography, and preparative TLC were used to further purify the crude hexane. The fractions obtained using the methods described above were analysed using GC-MS. The disc diffusion method was used to test the antibacterial activity of the hexane extract against two bacterial pathogens.

Results: Based on peak area percentage, retention time, and structure, eleven components were identified from crude hexane extract, eight components from Fraction 16c, and three components from Fraction 16d using GC-MS analysis.