Potential of Bioactive Compounds of Holothuria atra- Associated Bacteria as a Raw Material in Bioindustry

Abstract

Sea cucumbers have bioactive compounds with health efficacies. These active compounds can be used as raw material products for marine aquaculture. This study aims to determine the potential of sea cucumber intestine-associated bacteria as a product of bioindustry materials through investigation of their characteristics using the GC-MS method. The solid-liquid method is used in the extraction process. Open Column Chromatography (OCC) is used in the fractionation process. Anti-bacterial sensitivity test was carried out using the Kirby-Bauer agar diffusion method. This study found 26 bacterial isolates resulting from the isolation of black sea cucumber symbionts (Holothuria atra). Of the 26 bacterial isolates, 9 bacterial isolates were found to have antibacterial activity against MDR bacteria. Two isolates were selected from the 9 test results, namely T.1.2 which formed a 7.3 mm zone of inhibition against Escherichia coli and 7.8 mm against Staphylococcus aureus and T.1.13 which was only active against Staphylococcus aureus with an inhibition zone of 8.2 mm. Identification of selected isolates showed that isolate T.1.2 matched with Bacillus manliponensis species with 97% homology and that isolate T.4.1.25 matched with Bacillus oceanisediminis with 95% homology. GC-MS analysis found Methyl hexadecanoate/methyl palmitate, Bis (2-ethylhexyl) -1,2-benzene dicarboxylate, 9-Octadecenal, Glycerol-1,3-dihexadecanoate and Diisooctyl-1,2-benzene dicarboxylate in sea cucumber intestine-associated bacteria. Diisooctyl-1,2-benzene dicarboxylate can be used as raw materials for plastic which is usable as raw materials for bioindustry. The study concluded that the bioactive compounds of sea cucumber intestine-associated bacteria found in H. atra are useful as a raw ingredient of marine aquaculture products because they contain natural ingredients for plastic raw materials.