Isolation and Molecular Characterization of BCSP-31 Gene of Brucella abortus, a Significant Tool in Making Marker and DNA Vaccines from Cases of Bovine Abortion in an Organised Dairy Farm in India

Authors

  • Chhabil Singh Central Military Veterinary Laboratory (CMVL), Meerut Cantt, Meerut (U.P.), India.
  • Surender Kumar Central Military Veterinary Laboratory (CMVL), Meerut Cantt, Meerut (U.P.), India.
  • S. B. R. Sagi Central Military Veterinary Laboratory (CMVL), Meerut Cantt, Meerut (U.P.), India.
  • Vikas Thakur Central Military Veterinary Laboratory (CMVL), Meerut Cantt, Meerut (U.P.), India.
  • Amit Kumar Central Military Veterinary Laboratory (CMVL), Meerut Cantt, Meerut (U.P.), India.

DOI:

https://doi.org/10.9734/bpi/nuavs/v4/3669B

Keywords:

Brucella abortus, isolation, SAT, RBPT, PCR, sequencing, BCSP-31

Abstract

Brucellosis, characterized by abortion in females and orchitis in males along with infertility in both sexes, is an important zoonotic disease in many developing countries. Isolation of Brucella organism is considered as the gold standard diagnostic method for brucellosis since it is specific and allows biotyping of the isolate, which is relevant for control of brucellosis through vaccination. This chapter reports the isolation, identification and molecular detection of Brucella abortus. Brucella abortus was detected in four clinical samples by culture isolation and was subsequently confirmed by PCR. Molecular characterization of Brucella abortus BCSP-31 gene (protein which frequently elicits an immune response during infection and a significant tool in making marker and DNA vaccines) was successfully done in this chapter.

Published

2023-03-31

How to Cite

Chhabil Singh, Surender Kumar, S. B. R. Sagi, Vikas Thakur, & Amit Kumar. (2023). Isolation and Molecular Characterization of BCSP-31 Gene of Brucella abortus, a Significant Tool in Making Marker and DNA Vaccines from Cases of Bovine Abortion in an Organised Dairy Farm in India. Newest Updates in Agriculture and Veterinary Science Vol. 4, 62–70. https://doi.org/10.9734/bpi/nuavs/v4/3669B

Issue

Newest Updates in Agriculture and Veterinary Science Vol. 4

Section

Chapters