An Experimental Rat Model of Acute Pancreatitis and the Effect of Apigenin

Authors

  • Sovatzidis Apostolos Faculty of Medicine, Democritus University of Thrace, Alexandoupolis, 68100-Greece.
  • Oikonomou Panagoula Faculty of Medicine, Democritus University of Thrace, Alexandoupolis, 68100-Greece and  Department of Surgery, Faculty of Medicine, Democritus University of Thrace, Alexandroupolis-68100 Greece.
  • Nikolaidou Eirini Department of Plastic, Reconstructive and Hand Surgery & Burns ICU, General Hospital of Thessaloniki “G. Papanikolaou”, Thessaloniki, 57010-Greece.
  • Lambropoulou Maria Laboratory of Histology and Embryology, Faculty of Medicine, Democritus University of Thrace, Alexandroupolis, 68100-Greece.
  • Papachristou Fotini Laboratory of Experimental Surgery and Surgical Research, Faculty of Medicine, Democritus University of Thrace, Alexandroupolis, 68100-Greece.
  • Papalois Apostolos Educational and Research Center ELPEN, 19009- Greece.
  • Tsalikidis Christos Faculty of Medicine, Democritus University of Thrace, Alexandoupolis, 68100-Greece and Department of Surgery, Faculty of Medicine, Democritus University of Thrace, Alexandroupolis-68100 Greece.
  • Tsaroucha K. Alexandra Faculty of Medicine, Democritus University of Thrace, Alexandoupolis, 68100-Greece and Department of Surgery, Faculty of Medicine, Democritus University of Thrace, Alexandroupolis-68100 Greece and Laboratory of Experimental Surgery and Surgical Research, Faculty of Medicine, Democritus University of Thrace, Alexandroupolis, 68100-Greece.

DOI:

https://doi.org/10.9734/bpi/nhmmr/v11/6125F

Keywords:

Apigenin, rat, HMGB1, interleukin-6, interleukin-18, pancreatitis, resistin

Abstract

The aim of the study was to investigate the effect of apigenin (AP) on acute pancreatitis (ACP) by measuring the values of interleukin-6 (IL-6), interleukin-18 (IL-18) and resistin (R) in serum and CD45 as well as high mobility group box 1 protein (HMGB1) in intestinal tissue using a rat experimental model. 126 male Wistar rats were used, divided in three groups: Sham (S), Control (C) and Apigenin (Ap). The S group underwent laparotomy and closure of the abdominal wall. The C group underwent ligation of the pancreatic duct to induce acute pancreatitis. The apigenin group after induction of ACP, received AP. Blood and intestinal tissue were collected. Following AP administration, IL-6 and IL-18 in serum decreased as well as HMGB1 and CD45 in intestinal tissue. In contrast R remained at low levels in the Ap group. In a bilio-pancreatic duct ligation experimental model of ACP, AP treatment appears to protect intestinal and serum parameters, lowering the severity of ACP. As a result, giving this substance to humans could reduce the damage to the organs affected in the early stages of ACP.

Published

2022-06-10

How to Cite

Sovatzidis Apostolos, Oikonomou Panagoula, Nikolaidou Eirini, Lambropoulou Maria, Papachristou Fotini, Papalois Apostolos, … Tsaroucha K. Alexandra. (2022). An Experimental Rat Model of Acute Pancreatitis and the Effect of Apigenin. New Horizons in Medicine and Medical Research Vol. 11, 103–118. https://doi.org/10.9734/bpi/nhmmr/v11/6125F

Issue

New Horizons in Medicine and Medical Research Vol. 11

Section

Chapters