Rutin Attenuates Symptoms Associated with Endotoxemia by Regulating LPS-Induced Pro-Inflammatory Cytokines

Abstract

Background: Rutin, a natural flavonoid compound widely distributed in buckwheat, vegetables and fruits, is a well-known for a variety of biological activities and pharmacological effects.

Objectif: The present study aimed to explore whether Rutin could attenuate lipopolysaccharide (LPS)-induced inflammatory responses in an experimental endotoxemia. 

Methodology: We investigated the possible effects of Rutin against LPS-induced endotoxemia model in vivo to determine whether Rutin could rescue mice from ensuing death after their exposure to LPS. Forty-eight mice were divided into four groups (n=12/group) and challenged with LPS (5–40 mg/kg) via a single intraperitoneal (IP) injection. The mice were observed for mortality twice a day for 7 days, and survival rates were recorded. Mice were treated with RT at different doses (60, 80 or 100 mg/kg), or vehicle (5% of DMSO in saline), by the intraperitoneal (i.p.) route. One hour later, animals were challenged with LPS (20 mg/kg). The responses of cytokines were also assessed in serum isolated from blood collected at 0, 2, 4, 6, 8, and 24 h after LPS administration of the mice.

Results: The study indicates that Rutin showed suppressive effects on TNF- \(\alpha\), IL-6, and IL-1 \(\beta\) production by LPS- challenged mice. Furthermore, RT protected mice by improving host survival against LPS challenge.

Conclusion: The findings demonstrate that rutin offers protection against LPS-induced endotoxemia and will be potentially useful as an adjuvant treatment for inflammatory diseases.