Phytochemical Profiling and GC MS Analysis of Solanum incanum L Leaves

Abstract

In this chapter, GC-MS and qualitative methods have both been used to assess the phytochemical components of Solanum incanum L leaves. The phytochemicals in the petroleum ether, methanol, and ethanol extract of Solanum incanum L leaves were studied using a gas chromatograph (GC) SHIMADZU QP2010 system and gas chromatograph interfaced to a mass spectrometer (GC-MS) outfitted with an Elite-1 fused silica capillary column. The compounds were separated by using helium as the carrier gas at a constant flow rate of 1.51ml/min. Software called GC MS solution ver. 2.53 was used to manage mass spectra and chromatograms. According to preliminary research, there are carbohydrates, coumarins, flavonoids, phenols, steroids, and phytosteroids present. Twelve components were found in the petroleum ether, ethanol, and methanol extract of Solanum incanum L, according to the results of the GC-MS analysis. The major chemical constituents are Docosahexaenoic acid, 1,2,3 propanetriyl ether (Retention Time9.18), Ergoline-8-carboxylic acid, 10-methoxy-6-methyl-methyl ester, (8a) (Retention Time10.63), Ascorbic acid 2,6-dihexadecanoate (Retention Time11.9), Propanoic acid, 2-(3-acetoxy-4,4, 14 trimethylandrost-8-en-17-yl) (Retention Time 12.44). Understanding the nature of the active components in this plant begins with a GC-MS study of this kind. Future research and the search for novel medicines depend on the isolation of specific bioactive components and the biological activity of those components.