Recent study on Laboratory Development Cellular Immune Features and Immune Interference of Prototype Eschericia Coli and Pseudomonas Aeruginosa Combined Bacterins in a Lapin Model

Abstract

Bacterial vaccines, the bacterins are of both prophylactic and therapeutic potential s .Autogenous bacterins ,however, are of profound importance in certain clinical settings like complicated urinary tract infections. The aim of the present work was at development, cellular immune features and immune interference of combined E.coli and P,aeruginosa in rabbits. Single E.coli and single P. aeruginosa as well as balanced [1xE-1xP,2xE-2x P strength], and unbalanced [1xE-2xP, 2xE-1xP strength] heat killed bacterin combinations were prepared, developed and evaluated on laboratory scale. The developmental features were found; pure, safe, antigenic and immunogenic. These combined bacterins induced an increase in mitotic index of bone marrow cells, significant leukocyte inhibitory factors, increased spleen body index. Balanced one x and two x combined bacterins induced higher IL10 mean values than normal. 2x strength bacterin combinations initiate higher IL2 concentration mean values than single bacterin and control. Both of the unbalanced bacterin combinations were rising up the TNF alpha concentration means than that of single bacterin and control. In practical sense, the immune interference in rabbits primed with the study bacterin combination lead to, either of three results as; one damp the other, one enhance the other and one doesn’t affect the other. The immune interference appeared in the form of; one enhance the other like that of IL2 and IL10 cytokine responses. The present findings are being novel in cases of Pseudomonas lung and urinary tract infections as potential experimental therapeutic bacterin. As well as they may be of help in cases of cancer immunotherapy in man and laboratory animals.