Characterization and Preparation of New Injectable Regenerative Acellular Dermal Collagen Microparticles via a Designed Combined Procedure of Supercritical Fluids and Protease Treatments at Low Temperatures

Abstract

Tissue-engineering was important which combine medical applications and engineering materials. An extraction process was employed to remove the tissue and cellular molecules from porcine skin in this study via pretreatment of supercritical fluid a designed combined procedure of supercritical fluids of carbon dioxide and protease treatments at low temperatures. Varying porosities of the decellularized tissues were then created using protease reagent treatments.  The author studied characterizations of the designed decellularized scaffolds derived from porcine skins via supercritical fluid and protease reagent treatments.