Recent Advances in Dermatology Using DNA Aptamer “Aptamin C” Innovation: Oxidative Stress Prevention and Effect Maximization of Vitamin C through Antioxidation

Abstract

Introduction: By protecting cells from oxidative stress, Vitamin C (also known as L-ascorbic acid) plays an important role in reducing Reactive Oxygen Species (ROS) and promoting cell regeneration. Despite the fact that Vitamin C is widely utilised in cosmetic and medicinal industries, there is substantial evidence that it is easily oxidised by air, pH, temperature, and UV light when stored [1, 2].  Vitamin C deficiency diminishes its antioxidant activity and shortens the shelf life of products containing Vitamin C as a component.To combat vitamin C deficiency, we created Aptamin C, a revolutionary DNA aptamer that maximises the antioxidant activity of vitamin C by attaching to the reduced form of the vitamin and delaying its oxidation.

Methods: ITC analysis was used to determine the Binding of Aptamin C to Vitamin C. 0.2 mM Vitamin C was administered 25 times in 2\(\mu\)l aliquots into the 1.8 ml sample cell containing Aptamin C at a concentration of 0.02 mM in an ITC experiment. Using the origin programme for ITC v.5.0, the data were fitted to a one-site binding isotherm.

Results:  In vitro and clinical testing were carried out to see how Aptamin C and the Vitamin C complex affected human skin.We discovered that a compound of Aptamin C and Vitamin C was considerably efficient in reducing wrinkles, lightening the skin, and increasing moisture. In a clinical trial, participants who were given the compound saw a significant reduction in skin irritation and itching.

Conclusion: These findings suggested that Aptamin C, a novel and revolutionary molecule, might be used as a crucial cosmeceutical agent for a variety of skin disorders.