Study on Simple Detection of Bacillus anthracis Spores by Precipitation Method with Goat Antibody Anti Anthrosa

Abstract

Background: Bacillus anthracis has the potential to be used as a biological weapon or as a form of bioterorism. Bacillus anthracis attacks are very lethal, and spore transmission is simple and inexpensive. The goal of this research was to detect Bacillus anthracis spores.

Methods: To encourage capsule formation, Bacillus anthracis isolates were cultured on serum agar and then sheep blood medium.

Spores which formed painted using the method of Schaefer and Fultton.  Bacillus anthracis spores were detected using precipitation and immuno-chromatography techniques.

Results: Bacillus anthracis spores are green, as seen by painting with the Schaeffer and Fulton method. A silver white tint resulted from a precipitation interaction between Bacillus anthracis spores and goat antibody anti anthrosa.

Anti-anthrosa goat antibody was used to identify Anthrosa of Bacillus anthracis spores using immuno-chromatography. The molecular weight of anthrosa is ±148 kDa.

Conclusions: Bacillus anthracis spores can be detected utilising the methods of precipitation and immuno-chromatography using goat antibody anti anthrosa. Anti-anthrosa antibodies from goats have been found to react favourably with Bacillus anthracis spores.