Standardization of In-vitro Regeneration Protocol in Gerbera jamesonii Bolus Ex Hooker F.

Authors

  • Deepa Bhatt Horticultural Biotechnology Laboratory, KNK-College of Horticulture, Mandsaur - 458001, RVS Agricultural University, Gwalior, MP, India.
  • M. K. Tripathi Horticultural Biotechnology Laboratory, KNK-College of Horticulture, Mandsaur - 458001, RVS Agricultural University, Gwalior, MP, India and Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.
  • M. Vidhya Sankar Department of Floriculture and Landscaping, KNK-College of Horticulture, Mandsaur - 458001, RVS Agricultural University, Gwalior, MP, India.
  • Sushma Tiwari Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.
  • Mohini Sharma Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.
  • Niraj Tripathi Directorate of Research Services, Jawaharlal Nehru Agricultural University, Jabalpur 482004, India.
  • Sharad Tiwari Biotechnology Centre, Jawaharlal Nehru Agricultural University, Jabalpur 482004, India.

DOI:

https://doi.org/10.9734/bpi/ctas/v7/2303B

Keywords:

Gerbera jamesonii, mature embryo culture, direct and indirect organogenesis, direct and indirect somatic embryogenesis and plantlet regeneration

Abstract

Introduction: The gerbera belong to the family Asteraceae is the chief cut flowers and ranks among the top ten cut flowers in the universe. For commercial propgation of this plant species, planting material is required on large-scale which requires the employment of plant tissue culture techniques for massive in vitro propagation.

Study Objectives: In this investigation, an effort was made to compute optimal concentration of plant growth regulators added in culture medium and optimize other physical factors exhibiting higher in vitro response by culturing mature embryo in vitro.

Results: Nutrient media MS3D.5B (MS + 3.0 mgl-1 2, 4 D + 0.5 mgl-1BA + 30.0 gl-1 sucrose + 7.5 gl-1 agar powder) evidenced more appropriate for callus initiation. Inoculation media MS2N.5iP/MS3N.5ip (MS + 2.0/3.0mg l-1 NAA + 0.5 mgl-12-ip + 30.0 gl-1 sucrose + 7.5 gl-1 agar) displayed higher in vitro response i.e., numbers of shoot proliferating explants and numbers of shoot (s) per explant. While, shoot of higher length was recovered on culture medium MSB/MS2B (MS + 2.0/3.0 mgl-1BA + 30.0 gl-1 sucrose + 7.5 gl-1 agar). Enhanced in vitro rooting response (root proliferating efficiency, numbers of roots and root of higher length) were documented on rooting medium MS.1IB (MS + 0.1 mgl-1IBA + 15.0gl-1 sucrose + 7.5 g l-1 agar). The regenerants were transferred to pots and hardened in Environmental Growth Cabinet and net House and subsequently shifted to field conditions efficaciously. Phenotypic normal plants were acquired.

Published

2022-05-09

How to Cite

Deepa Bhatt, M. K. Tripathi, M. Vidhya Sankar, Sushma Tiwari, Mohini Sharma, Niraj Tripathi, & Sharad Tiwari. (2022). Standardization of In-vitro Regeneration Protocol in Gerbera jamesonii Bolus Ex Hooker F. Current Topics in Agricultural Sciences Vol. 7, 105–121. https://doi.org/10.9734/bpi/ctas/v7/2303B

Issue

Current Topics in Agricultural Sciences Vol. 7

Section

Chapters