Plant Regeneration in Indian Mustard [Brassica juncea (Linn.) Czern & Coss]: Experimental Investigation

Authors

  • C. Shyam Department of Genetics & Plant Breeding, College of Agriculture, RVS, Agricultural University, Gwalior, 474002 M.P. India.
  • M. K. Tripathi Department of Genetics & Plant Breeding, College of Agriculture, RVS, Agricultural University, Gwalior, 474002 M.P. India and Department of Plant Molecular Biology & Biotechnology, College of Agriculture, RVS Agricultural University, Gwalior, 474002 M.P. India.
  • S. Tiwari Department of Plant Molecular Biology & Biotechnology, College of Agriculture, RVS Agricultural University, Gwalior, 474002 M.P. India.
  • A. Ahuja Department of Plant Molecular Biology & Biotechnology, College of Agriculture, RVS Agricultural University, Gwalior, 474002 M.P. India.
  • N. Tripathi Directorate of Research Services, Jawaharlal Nehru Agricultural University, Jabalpur 482004, India.
  • N. Gupta Department of Plant Molecular Biology & Biotechnology, College of Agriculture, RVS Agricultural University, Gwalior, 474002 M.P. India.

DOI:

https://doi.org/10.9734/bpi/ctas/v3/2118C

Keywords:

Indian mustard, callus, cell suspension culture, somatic embryogenesis, plantlet regeneration

Abstract

Brassica juncea is an important mustard species grown for edible oil in India. The current study establishes an efficient and reproducible plant regeneration protocol from callus employing mature cotyledons and seeds as explants and embryogenic cell suspension cultures derived from embryogenic friable calli. MS basal media enriched with 3.0 mgl-1 2, 4-D produced the best callus induction. Supplementation of 0.5 mgl-1 BAP in combination with 0.5-1.0 mgl-1 2,4-D resulted in further regeneration via embryogenesis/ organogenesis. The superiority of mature seeds as explants has been demonstrated, implying that this explant has greater morphogenic potential. Furthermore, the genotype, type (s), and relative concentrations and combinations of plant growth regulators all had a substantial impact on the success of plantlet regeneration. In the future, these techniques could be used for genetic transformation and in vitro selection of Indian mustard elite genotypes.

Published

2021-10-30

How to Cite

C. Shyam, M. K. Tripathi, S. Tiwari, A. Ahuja, N. Tripathi, & N. Gupta. (2021). Plant Regeneration in Indian Mustard [Brassica juncea (Linn.) Czern & Coss]: Experimental Investigation . Current Topics in Agricultural Sciences Vol. 3, 120–135. https://doi.org/10.9734/bpi/ctas/v3/2118C

Issue

Current Topics in Agricultural Sciences Vol. 3

Section

Chapters