The Occurrence of the Pseudophyllidean, Spirometra Species, in a Tanzanian Dog with a Molecular Approach

Authors

  • N.J. Kavana Department of Veterinary Microbiology and Parasitology, Faculty of Veterinary Medicine, Sokoine University of Agriculture, P.O. Box 3019, Morogoro, Tanzania and Department of Microbiology and Parasitology, Faculty of Medicine, St. Francis University College of Health and Allied Sciences Ifakara, Tanzania.
  • C.J. Kasanga Department of Veterinary Microbiology and Parasitology, Faculty of Veterinary Medicine, Sokoine University of Agriculture, P.O. Box 3019, Morogoro, Tanzania.
  • A.A. Kassuku Department of Veterinary Microbiology and Parasitology, Faculty of Veterinary Medicine, Sokoine University of Agriculture, P.O. Box 3019, Morogoro, Tanzania.
  • S. Parthasarathy Department of Parasitology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
  • Y.L. Lau Department of Parasitology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
  • M.Y. Fong Department of Parasitology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
  • R. Mahmud Department of Parasitology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.

DOI:

https://doi.org/10.9734/bpi/crpbs/v6/3107

Keywords:

Spirometra, PCR, cloning, sequencing

Abstract

Cestodes of the genus Spirometra are multi-host parasites that are causative agents of spirometrosis in domestic and wild carnivores and sparganosis in humans. In domestic animals, the infection is usually asymptomatic. This study investigated the Spirometra species obtained from a domestic dog from Tanzania. The polymerase chain reaction (PCR) was used for molecular identification of Spirometra. The amplicons derived from genomic DNA samples from a single adult worm were subjected to sequencing and compared with that of S. erinaceieuropaei. The gene arrangement in the mt genome sequences of S. erinaceieuropaei from Tanzania is identical. The identity of the mt genomes was 100% between S. erinaceieuropaei from Tanzania. The result of this study does not support earlier conclusions that Tanzanian Spirometra sp. is S. theileri. This is the first report of S. erinaceieuropaei in dogs from Tanzania.

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Published

2024-12-07

How to Cite

N.J. Kavana, C.J. Kasanga, A.A. Kassuku, S. Parthasarathy, Y.L. Lau, M.Y. Fong, & R. Mahmud. (2024). The Occurrence of the Pseudophyllidean, Spirometra Species, in a Tanzanian Dog with a Molecular Approach. Contemporary Research and Perspectives in Biological Science Vol. 6, 65–75. https://doi.org/10.9734/bpi/crpbs/v6/3107

Issue

Contemporary Research and Perspectives in Biological Science Vol. 6

Section

Chapters