Cutting-edge Research in Agricultural Sciences Vol. 13

Abstract

Inter simple sequence repeat (ISSR) markers are being routinely used to detect genetic polymorphisms. Study employed ISSR markers to ascertain genetic diversity in 13 accessions of chickpea including cultivated and wild. Among all these anchored ISSR primers tested, pentanucleotide repeat primer UBC-879 produced better amplification patterns. A total of 150 bands were amplified in a molecular weight range of 100-2000 bps revealing an average of 21.4 bands per primers and 1.64 bands per primer per genotype. The repeats (GA)8C, (AG)8YT, (GA)8YC, (AG)8C, (GTT)6 and (GT)8YC give least amplification.