Determination of Molecular Characterization of Endangered Medicinal Plant Hedychium coronarium from Eastern India

Abstract

In the present report, two PCR based molecular markers like RAPD and ISSR has been used to characterize different populations of Zingiberaceae. Molecular characterization of Hedychium coronarium from four different populations in Odisha using nine ISSR and fifteen RAPD markers to quickly and efficiently determine the closeness of species and hybrids. A dendrogram was constructed through sequential agglomerative hierarchial and nested (SHAN) clustering and un-weighted pair group method with arithmetic mean (UPGMA) analysis using Jaccard’s similarity coefficient of combined markers using this particular species. Two major clusters were found i.e., cluster-I (Malkangiri-1, Phulabani-1, Phulabani-3, Malkangiri-2, Khurda-1, Khurda-2, Khurda-3, Angul-3, Angul-1, Angul-2 and Phulabani-2) and cluster-II (Malkangiri-3). The clustering pattern also revealed moreover the extent of genetic similarity between germplasms collected from four different regions population. The full potential of this technique will be realised for the identification and tagging of important novel genes in previously unexplored taxa, facilitating the improvement of desired Zingiberaceae taxa. The findings would be of immense enough significance for complementing the strategies of conservation and characterization of these important taxa of Zingibearceae following modern biotechnological approach. The competence of ISSR in clonal fidelity assessment on Allium and Aloe was established successfully.