Application of Nano-doped Zinc Oxide on the Retina of Diabetic Rats as Protective agent against Oxidative Stress

Abstract

The development of diabetic retinopathy (DR) is primarily caused by oxidative stress. Therefore, the aim of this work conducted to evaluate the protective effect of zinc oxide nanoparticles (ZnONPs) doped with chlorine (Cl: ZnO), and fluorine (F: ZnO) on retinal oxidative stress in diabetic rats. The characterizations (FTIR, X-ray, SEM, and BET) of Cl: ZnO and F: ZnO were determined. Blood glucose, insulin, sAST, and sALT were measured. Also, TBA in the retinal and liver, and GSH in the retinal were assessed. Results of TEM showed that the average particle size of Cl: ZnO and F: ZnO are 59.3 and 17.7 nm, respectively. Serum insulin was decreased and serum glucose was increased significantly (p \(\leq\) 0.05) and depleted both retinal GST and retinal and liver TBA contents 3 days after treatment with STZ compared to other groups. Low and high doses of STZ + Cl: ZnO and STZ + F: ZnO in STZ-administered animals, ZnO therapy decreased serum glucose levels, reversed the depletion effect on retinal GSH levels; and decreased spikes in TBA levels in the livers and retinas. The findings look at the useful effects of a high dose of Cl: ZnO and F: ZnO in preventing retinal damage in diabetic rats and oxidative stress in hyperglycemia.