Specific Activity of Amylase and Activation Energy Required for the Activity and Q10 Values in Different Temperature Regimes in Telescopium telescopium

Abstract

This chapter aimed to determine the specific activity of amylase, activation energy required for the activity and Q10 values in different temperature regimes.  Enzyme alpha amylase was purified to the electrophoretic homogeneity from hepatopancreas of Telescopium telescopium by ammonium sulphate fractionation procedures, dialysis and chromatographic procedure. Specimens of Telescopium telescopium were collected from Mandovi estuary, Choraon Islands, Goa during 2004 to 2006. Each animal was opened with the help of bone cutter aseptically. The present investigation shows that the energy of activation nearly remained the same at 40-60oC but for a temperature range of 30-40oC it was second highest to that obtained at 10-20oC. Purified enzyme had the activity equivalent to 42.12 \(\mu\) mol/mg/min. Samples were collected from Choraon Islands of Goa State during 2004-2006. Energy of Activation was maximum between temperatures \(10^{\circ}\)C - \(20^{\circ}\)C,187.38 ±10.43 J/mol; at \(60^{\circ}\)C to \(70^{\circ}\)C it was equivalent to about 154 J/mol. Q10 value was highest at a temperature range of \(10^{\circ}\)C to \(20^{\circ}\)C and the enzyme velocity elevated by a factor of 0.065 ± 0.009. The energy of activation for amylases on an average is 163.83±3.33 Jules/mol and this is probably the first report on energy of activation requirement of amylases from any mollusk studied so far. As the temperature range was elevated beyond \(20^{\circ}\)C up to \(70^{\circ}\)C, the lowest Q10 value was obtained at a temperature range of \(60^{\circ}\)C to \(70^{\circ}\) and it was equivalent to 0.001 ±0.00. The results of the experiment indicate that the successive rise in temperature the Q10 values of \(\alpha\)-amylases of Telescopium telescopium lower subsequently.