Design, Synthesis, and Evaluation of Nucleoside Analogues as Potent Inhibitors of the GOT Enzyme

Abstract

Two primary sections were investigated in this study. The synthesis of new Mannich derivatives of the nucleoside cytidine is the main topic of the first section. This is accomplished by reacting cytidine with secondary amines, namely morpholine, pyrazine, pyridine, and pyrrolidine, while formaldehyde is present. Nucleoside analogues (1, 2, 3, and 4) are produced by this procedure, and their structural integrity is then verified by FT-IR spectroscopy, ¹³C NMR, and ¹H NMR characterization. The second section assesses how these synthetic substances affect the activity of glutamate oxaloacetate transaminase (GOT) in human serum samples. Under the same circumstances, enzyme activity is assessed both with and without the inhibitors. The most effective inhibitors are found, the inhibition mechanism is ascertained, and the inhibition ratios are computed. The findings emphasize the potential of the synthesized nucleoside analogues as enzyme inhibitors by showing that they have strong inhibitory effects against GOT.