Assessment of Disease Management by Vaccination using Escherichia coli Antigens

Abstract

The rise in pathogen resistance is a serious concern for health care in the twenty-first century, and effective vaccines to prevent life-threatening diseases are urgently needed. In order to uncover new antigens with diagnostic and vaccine potential, pathogens have been studied for their potential utility as therapeutic or preventive vaccines. The present investigation was done with five different type of antigens produced from single pathogen. It also identifies the immune complex and DNA antigen as most suitable for vaccine development against pathogens. Antigens may vary within the host during the course of infection or antigenic types of parasites in the population. Antigenic variation is an important mechanism used by pathogenic microorganism for escaping and neutralizing activities of the antibody. Immune complexes and DNA antigen serve as vaccines, and it can be used to develop multi antigen. Furthermore, it is also easy to produce in a large scale. Vaccination is still the best way for prevention of bacterial diseases. The conditions for the preparation of antigens of intact natural composition and conformation from Escherichia coli (whole cell and heat killed), was determined using Swiss albino mice (Balb/C) as experimental species. A novel approach for vaccine design and production is discussed. Components leading to the pathology, together with the prevention of the escape mechanism involved by pathogens are the major idea of vaccine technology.