Development of Multivariate Calibration Technique for the Spectrophotometric Quantification of Ivermectin in Pharmaceutical Formulation

Abstract

Objective: The current abstract employs a multivariate calibration technique to quantify ivermectin in pharmaceutical dosage form.

Methods: The multivariate calibration technique relies on linear regression equations to correlate the relationship between concentration and absorbance at seven different wavelengths.  The \(\lambda_{max}\) of ivermectin was found to be 254 nm. The findings were statistically analysed. This statistical approach yields the best results by eliminating fluctuations caused by instrumental or experimental conditions.

Results: The developed method was validated in accordance with ICH guidelines and was found to be simple, linear, accurate, precise, and reproducible. The method was found to be linear over a concentration range of \(5-15 \mu\\g/mL\) with a correlation coefficient \((r^2)\) value of about 0.9998. The limit of detection and quantification were found to be 0.029 and \(0.087 \mu\\g/mL\), respectively. Intraday and interday precision were found to have percentage relative standard deviations of 0.473-1.373 and 0.301-1.617, respectively. The percentage recovery was found within the range of \(97.60-101.80\%w/w\).

Conclusion: The findings show that a simple, linear, precise, accurate, sensitive, and reproducible multivariate calibration technique for the quantification of ivermectin in bulk and pharmaceutical formulation has been devised and validated.