Low and High Molecular Weight Chitosan Analogues of Imidazole-2-Thiosemicarbazones and Their Copper(II) Complexes: Synthesis, Characterization, and Antitumorigenic Activity in vitro

Authors

  • Hari Sharan Adhikari Institute of Engineering, Pashchimanchal Campus, Department of Applied Sciences, Tribhuvan University, Pokhara, Nepal.
  • Aditya Garai Department of Inorganic and Physical Chemistry, Indian Institute of Science, Bangalore-560012, India.
  • Chetana Khanal Central Department of Biotechnology, Tribhuvan University, Kathmandu, Nepal.
  • Rameshwar Adhikari Research Centre for Applied Science and Technology (RECAST), Tribhuvan University, Kathmandu, Nepal and Central Department of Chemistry, Tribhuvan University, Kathmandu, Nepal.
  • Paras Nath Yadav Central Department of Chemistry, Tribhuvan University, Kathmandu, Nepal.

DOI:

https://doi.org/10.9734/bpi/cacs/v8/15406D

Keywords:

Antitumorigenic activity, Chitosan thiosemicarbazones, Imidazole-2-carboxaldehyde, MDCK cell line

Abstract

The current study aims at comprehensive study of the effects of Mw and DDA of chitosan on antitumorigenic activity of chitosan thiosemicarbazones and their copper(II) complexes. It has been anticipated that the antitumorigenic activity be enhanced with the synergistic effects upon the functionalization of chitosan as chitosan thiosemicarbazones and the complexes could show more potent antitumorigenic activity. Chitosan oligosaccharide and high molecular weight crab shell chitosan were functionalized as imidazole-2-carboxaldehyde chitosan thiosemicarbazones and their copper(II) complexes were synthesized. The synthesized compounds were characterized by FT-IR, 13C NMR, EPR spectroscopy, powder X-ray diffraction (PXRD) analysis, elemental analysis and magnetic susceptibility measurements. The low molecular weight chitosan thiosemicarbazones showed higher in vitro inhibitory activity as studied by MTT assay against the tumorigenic epithelial Madin-Darby Canine Kidney (MDCK) cell line than the corresponding high molecular weight chitosan derivative. The antitumorigenic enhancement upon the complex formation was revealed by the better inhibitory activity of copper(II) chitosan thiosemicarbazone chelates.

Published

2022-02-07

How to Cite

Hari Sharan Adhikari, Aditya Garai, Chetana Khanal, Rameshwar Adhikari, & Paras Nath Yadav. (2022). Low and High Molecular Weight Chitosan Analogues of Imidazole-2-Thiosemicarbazones and Their Copper(II) Complexes: Synthesis, Characterization, and Antitumorigenic Activity in vitro. Challenges and Advances in Chemical Science Vol. 8, 121–137. https://doi.org/10.9734/bpi/cacs/v8/15406D

Issue

Challenges and Advances in Chemical Science Vol. 8

Section

Chapters