Study on Immobilization of Formate Dehydrogenase on Polyethyleneimine Modified Carriers for the Enhancement of Catalytic Performance

Authors

  • Meng Ya Guo School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Liangxiang Higher Education Park, Fangshan District, Beijing 102488, P. R. China.
  • Ting Ting Zhai School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Liangxiang Higher Education Park, Fangshan District, Beijing 102488, P. R. China.
  • Cai Hong Wang School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Liangxiang Higher Education Park, Fangshan District, Beijing 102488, P. R. China.
  • Zi Hui Meng School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Liangxiang Higher Education Park, Fangshan District, Beijing 102488, P. R. China.
  • Wen Fang Liu School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Liangxiang Higher Education Park, Fangshan District, Beijing 102488, P. R. China.

DOI:

https://doi.org/10.9734/bpi/cacs/v8/1593B

Keywords:

Formate dehydrogenase, CO2 conversion, formate, polyethylenimine, polyethylene membrane, silica, enzyme immobilization, synergistic effect

Abstract

CO2 hydrogenation represents one important application way that not only removes CO2 from the atmosphere, but also generates high value-added chemicals.   In the field of CO2 adsorption, polyethyleneimine (PEI) is a popular modifier, however it's rarely utilised in the immobilisation of enzymes that catalyse CO2 conversion. In this study, formate dehydrogenase (FDH) was immobilised on the surface of PEI-modified polyethylene membranes or silica microspheres and then used in the CO2 to formate process. The catalytic performance of immobilised FDH was 4.3 and 7.4 times that of free enzyme after optimising the immobilisation settings, owing to the carrier's promoting impact. FDH linked to polydopamine/PEI-SiO2 has higher activity and stability than biocatalytic membrane.

Published

2022-02-07

How to Cite

Meng Ya Guo, Ting Ting Zhai, Cai Hong Wang, Zi Hui Meng, & Wen Fang Liu. (2022). Study on Immobilization of Formate Dehydrogenase on Polyethyleneimine Modified Carriers for the Enhancement of Catalytic Performance. Challenges and Advances in Chemical Science Vol. 8, 1–11. https://doi.org/10.9734/bpi/cacs/v8/1593B

Issue

Challenges and Advances in Chemical Science Vol. 8

Section

Chapters