The Importance of Fresh Consumption of Citrus Juices

Abstract

Two different reversed-phase high performance chromatographic methods for simultaneous determination of naringin, hesperidin and organic acids in natural and commercial citrus juices were developed. The separation was achieved on C18 column using an acetonitrile: water: formic acid (21:78.8:0.2) solution at pH 2.5 as a mobile phase under isocratic conditions for naringin and hesperidin. The analysis was run at a flow rate of 1 mL.min^-1 with a 15 min total run time at room temperature. The detection wavelength was set at 280 nm. The chromatographic separation of organic acids was performed in a single run by using isocratic mobile phase consisting of 10 mM KH₂PO₄ (pH=2.2) at room temperature, with flow rate of 1 mL.min^-1 within 10 min on C18 column. An ultraviolet absorption at 210 nm and 245 nm was monitored. Linearity of calibration, accuracy, and  precision were examined as parts of the method validation. The optimized method was applied to analysis of natural and commercial citrus juices. Commercial fruit juices contained lower amount of naringin, hesperidin and organic acids compared with natural citrus juices.