Simultaneous Determination of Ofloxacin and Tinidazole from Pharmaceutical Dosage Formulation by Two Spectrophotometric Methods

Abstract

Two simple, sensitive, accurate, precise, rapid and economical methods were developed for the estimation of Ofloxacin and Tinidazole from combined tablet dosage form. First method is based simultaneous equation and second method is based on ?analysis (absorbance ratio method). Ofloxacin and Tinidazole show  at 300 nm and 275 nm in 0.  Methanolic?HCI (20:80) respectively. The linearity was obtained in the concentration ranges of  for Ofloxacin and  for Tinidazole with Regression Coefficient (R²) greater than 0.999. In the first method concentration and subsequently amount of drug determined by using simultaneous equations and in second method concentration and amount of drug determined by using ratio of absorbance at Iso?absorptive point (which was found to be 286 nm) and at  of one of the drugs. The results of analysis have been validated statistically and subsequently by assay and recovery studies.