Determination of Polyphenols in Ilex kudingcha and Insect Tea (Leaves Altered by Animals) by Ultra-high-performance Liquid Chromatography-Triple Quadrupole Mass Spectrometry (UHPLC-QqQ-MS) and Comparison of Their Anti-Aging Effects

Authors

  • Jianfei Mu Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing, China and College of Food Science, Southwest University, Chongqing, China.
  • Fuping Yang Tuberculosis Section III, Chongqing Public Health Medical Treatment Center, Chongqing, China.
  • Fang Tan Department of Public Health, Our Lady of Fatima University, Valenzuela, Philippines.
  • Xianrong Zhou Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing, China.
  • Yanni Pan Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing, China and Department of Food Science and Biotechnology, Cha University, Seongnam, South Korea.
  • Xingyao Long Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing, China and Department of Food Science and Biotechnology, Cha University, Seongnam, South Korea.
  • Xin Zhao Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing, China.

Keywords:

ilex kudingcha, insect tea, polyphenols, aging, UHPLC-QqQ-MS

Abstract

Ilex kudingcha C.J. Tseng tea and insect tea, as traditional Chinese teas, are favored for their original craftsmanship, unique flavor, and biological functionality. In this study, ultra high-performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ-MS) was used to analyze the bioactive components of the extracts of Ilex kudingcha and insect tea, and D-galactose-induced aging mice were used to compare the in vivo anti-aging effects of Ilex kudingcha and insect tea extracts. The results were remarkable, UHPLC-QqQ-MS analysis showed that ITP contains 29 ingredients, while IKDCP contains 26 ingredients. However, due to the large differences in the content of the main chemical components in IKDCP and ITP, the effects are equally different. At the same time, the in vivo research results suggesting that the anti-aging effects of IKDCP and ITP (500 mg/kg) include the regulation of viscera indices of major organs; improvement in liver, skin, and spleen tissue morphology; decreased production of inflammatory cytokines; up regulation of SOD, CAT, GSH, GSH-PX, and T-AOC and down regulation of NO and MDA levels in serum and liver tissue; reductions in the concentration of pro-inflammatory factors, and increases in the concentration of anti-inflammatory factor. RT-qPCR and western blot assay also showed that IKDCP and ITP affect anti-aging by regulating the gene and protein expression of GSH-PX, GSH1, SOD1, SOD2, and CAT. The overall results indicate that ITP is more effective in treating oxidative damage in aging mice induced by D-galactose. Thus, ITP appears to be an effective functional drink owing to its rich nutritional components and anti-aging activities.

Published

2021-05-06

How to Cite

Jianfei Mu, Fuping Yang, Fang Tan, Xianrong Zhou, Yanni Pan, Xingyao Long, & Xin Zhao. (2021). Determination of Polyphenols in Ilex kudingcha and Insect Tea (Leaves Altered by Animals) by Ultra-high-performance Liquid Chromatography-Triple Quadrupole Mass Spectrometry (UHPLC-QqQ-MS) and Comparison of Their Anti-Aging Effects. Bioactivity of Chinese Insect Tea, 42–65. Retrieved from https://stm.bookpi.org/BCIT/article/view/954

Issue

Bioactivity of Chinese Insect Tea

Section

Content