Growth Inhibition of Cancer Cell Line Using Fungal Secondary Metabolite

Authors

  • Batol Imran Dheeb Department of Pathological Analysis, College of Applied Sciences, University of Samarra, Samarra, Iraq.
  • Humam Saadi Hussein Department of Biology, College of Sciences, University of Tikrit, Tikrit, Iraq.
  • Akeel Hussain Ali Al Asi Department of Biotechnology, College of Applied Sciences, University of Samarra, Samarra, Iraq.
  • Asmaa Abdukreem Alwan Department of Biotechnology, College of Applied Sciences, University of Samarra, Samarra, Iraq.
  • Hayder Mudheher Abbas Department of Biology, College of Sciences, University of Tikrit, Tikrit, Iraq.
  • Fatiha El Hilali Medico-Surgical, Biomedicine and Infectiology Research Labratory, The Faculty of Medicine and Pharmacy of Laayoune & Agadir, Lbnou Zohruniversity, Agadir, Morocco.
  • Muhannad I. Massadeh Department of Biology and Biotechnology, The Hashemite University, Zarqa, Jordan.
  • Abbas Fadhel Hamid Mistry of Health, Samaraa General Hospital, Iraq.

DOI:

https://doi.org/10.9734/bpi/arbs/v3/7206A

Keywords:

Gliotoxin, hemolysin, protease, melanin, cytotoxic activity

Abstract

The purpose of this study was to compare the cytotoxic activity of the A. fumigaus virulence factors. Particularly in clinical isolates, Aspergillus fumigatus generates a number of toxic compounds, proteinaceous hemolysin, protease, and pigments like melanin that seem to act synergistically and additively on cells. In this work, gliotoxin, hemolysin, protease, and melanin were utilized in an experimental model to evaluate their cytotoxic activity by analyzing their effect on the Hep G2 cell line. Exposure times of 24 hours at three different doses of each component were employed, with triplicates of each concentration. A toxic effect was evident with a significant difference at the level of probability (p 0.05) for each purified compound, and this effect was contrasted across different concentrations for each purified compound. Growth inhibition of the Hep G2 cell line was gradually increased with the increase of compound concentration.

Published

2023-08-14

How to Cite

Batol Imran Dheeb, Humam Saadi Hussein, Akeel Hussain Ali Al Asi, Asmaa Abdukreem Alwan, Hayder Mudheher Abbas, Fatiha El Hilali, … Abbas Fadhel Hamid. (2023). Growth Inhibition of Cancer Cell Line Using Fungal Secondary Metabolite. Advanced Research in Biological Science Vol. 3, 177–187. https://doi.org/10.9734/bpi/arbs/v3/7206A

Issue

Advanced Research in Biological Science Vol. 3

Section

Chapters