Development and Validation of a HPLC Method for the Simultaneous Estimation of Simvastatin and Metformin

Abstract

Simvastatin is one of the well-known statins which is a HMG-COA reductase inhibitor. Metformin hydrochloride is a well- known oral antihyperglycemic drug. The present work has designed and validated a precise and accurate reverse phase liquid chromatographic method for the simultaneous estimation of simvastatin and metformin. This is because the reversed phase HPLC method is the most suitable for assessing both ingredients at the same time. The method was created using a Jasco Auto sampler HPLC with 50mM Sodium Dihydrogen Phosphate Monohydrate at pH 3.0 and Acetonitrile (30:70 v/v). UV-visible detectors were used for the analysis, and 235 nm was used to monitor the eluents. Simvastatin and metformin were shown to have retention times of 7.650 and 2.858 minutes, respectively. The approach was validated in terms of linearity, robustness, precision, and accuracy. The planned technique for the simultaneous quantitative determination was implemented effectively.