Determining the Antibacterial Effect of the Leaf Extract of Buchholzia coriacea on Nasal Isolates

Abstract

The present research examined the antibacterial activity of nasal isolates on Buchholzia coriacea (Wonderful Kola) leaf extract. There is a lengthy history of traditional medicine. It is the whole of the knowledge, skill, and behaviors based on ideas, beliefs, and experiences that are inherent to many cultures, whether or not they can be explained, and employed in the preservation of health as well as in the avoidance, diagnosis, improvement, or treatment of physical and mental disease. One hundred pupils at the Delta State Polytechnic in Ogwash-uku, Delta State, were tested for nasal isolates. Using the agar well diffusion method, the antibacterial activity of the methanolic extract of the leaves was evaluated against nasally isolated microorganisms (E. coli, Citrobacter species, Klebsiella species, and Proteus species). Inhibition zones and Minimum Inhibitory Concentration (MIC) were used as indicators of antimicrobial activity. The extracts inhibited the growth of the bacterial isolates in a concentration dependent manner with MICs of 39.81mg/ml, 69.18mg/ml, 79.43mg/ml and 97.7mg/ml (E. coli, Citrobacter species, Klebsiella species and Proteus species) respectively. Phytochemical screening reveals the presence of secondary metabolites; tannins, saponins, cardiac glycosides, purines, reducing sugars, flavonoids, steroids, alkalloids and phlobatannins. The result indicates promising antibacterial potential of B. coriacea leaf extracts and hence could be considered for pharmaceutical and medicinal purposes. Characterization of the bioactive components of B. coriacea leaves could be used in the development of drugs for the treatment of bacterial related infections.